The aim of the present study was to investigate differences in the acoustic characteristics of the voice between adolescent actors and non-actors. The experimental sample consisted of 10 actresses and 10 actors while the control sample included 13 girls and 14 boys. Phonation of the vowel /a/, spontaneous speaking and oral reading provided a set of acoustic variables (fundamental frequency, jitter, shimmer, speaking and reading ranges); t test showed statistically significant differences between actresses and non-actresses in speaking range, reading F₀ maximum, and reading range, whereas between actors and non-actors the difference was found in reading range only. The results showed that drama in education without systematic voice training had no effects in terms of acoustic characteristics of the voice.