Nanostructured alpha-nickel hydroxide (a-Ni(OH) 2 ) immobilized on a Fluorine-doped Tin Oxide (FTO) surface was explored for the construction of hydrogen peroxide amperometric Flow Injection Analysis (FIA) sensors. Their notable electrocatalytic activity and heterogeneous electron-transfer rate were confirmed by the appearance of a broad and intense peak associated with the oxidation of hydrogen peroxide and the enhancement of sensibility in hydrodynamic conditions. The a-Ni(OH) 2 electrodes exhibited a broad dynamic range (5 10 À6 to 1 10 À3 mol L À1 ), low detection limit (2 10 À7 mol L À1 ), good repeatability (RSD = 1.29 % for 20 successive analyses), and a sensitivity greater than 500 mA mmol À1 L À1 cm À2 .