Summary Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P 2 ] is a low abundant membrane lipids essential for plasma membrane function. In plants, mutations in PI4P 5-kinases (PIP5K) suggest that PI(4,5)P 2 production is involved in development, immunity and reproduction. However, phospholipid synthesis is highly intricate. It is thus likely that steady-state depletion of PI(4,5)P 2 triggers confounding indirect effects. Furthermore, inducible tools available in plants, allow to increase but not decrease PI(4,5)P2, and no PIP5K inhibitors are available. Here, we introduce iDePP ( I nducible Dep letion of PI (4,5)P 2 in P lants), a system for the inducible and tunable depletion of PI(4,5)P 2 in plants in less than three hours. Using this strategy, we confirm that PI(4,5)P 2 is critical for various aspects of plant development, including root growth, root hair elongation and organ initiation. We show that PI(4,5)P 2 is required to recruit various endocytic proteins, including AP2, to the plasma membrane, and thus to regulate clathrin-mediated endocytosis. Finally, we uncover that inducible PI(4,5)P 2 perturbation impacts the dynamics of the actin cytoskeleton as well as microtubule anisotropy. Together, we propose that iDePP is a simple and efficient genetic tool to test the importance of PI(4,5)P 2 in given cellular or developmental responses, but also to evaluate the importance of this lipid in protein localization.