A significant therapeutic plant from the Malvaceae family is Abutilon hirtum (L.). It is frequently used in traditional medicine to treat a variety of illnesses. Rutin is a flavonoid molecule with commercial value that has anticancer, nutritional, and anti-ageing properties. This research project was carried out to evaluate the effect of abiotic elicitor Methyl Jasmonate (MeJA) at concentrations 0.0, 0.01, 0.02, 0.03 mmol.L-1 on accumulation of rutin by callus cultures  of  Abutilon hirtum L. after 30 days of growth on  Murashige and Skoog (MS) medium to which 2000 µg.L-1 2,4-Dichlorophenoxy acetic acid (2,4-D) + 500 µg.L-1 Kinetin (Kin) were added. The results showed that the best callus stems cultivated on MS growth medium fortified with 2000 µg.L-1 2,4-D + 500 µg.L-1 Kin, recorded the highest fresh weight of 2.887 g after four weeks. This induced callus was characterized by its friable texture. The data of rutin detection in cultures of callus obtained from stems explant of A. hirtum L., using High-Performance Liquid Chromatography (HPLC) indicated the presence of rutin in these cultures by comparison with the rutin standard samples containing a precisely known concentration of a substance for use in quantitative analysis.. Treatment with 0.03 mmol.L-1 of Methyl Jasmonate gave the best rutin amount of 5.606 mg.g-1 was much higher than the control treatment of 1.569 mg.g-1. These data clearly indicated that callus cultures are a potential continuous and constant source of rutin, as a secondary metabolite, and as an alternative to field plants.

Nanoscience science the studys the infinitesimal world, which is the world of atoms and molecules. The agricultural field faces many challenges, including climate change, the increase in the consumption of agricultural products and the reducing of the cultivated area, which requires the need to advance agricultural development to achieve economic and agricultural stability. Hence the importance of using nanotechnology and technology, which enables the development of modern methods in the possibility of finding and treating many agricultural problems. This is in addition to some of the main challenges related to the field of agriculture, including the low productive efficiency in the cultivated area, the large size of the uncultivated area, the loss of resources, including water, fertilizers and pesticides, and the loss of products. This is in addition to the food security of the developing population, which can be faced through the various applications of nanotechnology. Nanotechnology achieves remarkable achievement rates in community service and environmental development. Yellow corn production is considered one of the most important grain crops in Iraq to confront the basic needs of humans and animals.