The present study was carried out on 17 seeded strains of date palm "Phoenix dactylifera L." grown in Basrah region to investigate variations in protein pattern of leaflets and fruits among these strains. Cluster and principal component analysis were used to identify the degree of similarity and differences between date palm strains. The leaflet and fruit protein pattern on SDS-PAGE showed differences in band number, location, thickness and density leading to variations in protein quantity and quality among the studied strains.Cluster analysis data showed a high degree of similarity between leaflet and fruit samples of these strains recording an average similarity value of 0.76 and 0.67 for leaflet and fruit samples respectively. Cluster analysis also revealed that all strains occupied two main groups and then distributed into two subgroups under each main group. The principal component analysis showed that protein banding characters for leaflets and fruits could be used for identification of date palm seeded strains.
This study was conducted on shoot tip explants of date palm cv. Barhee to investigate effect of bacterial cell wall components on initial callus growth. Three concentrations of bacterial media ( 0.5 and 1.0 and 2.5) v/v were used for growing callus by tissue culture technique . MS media was served as a control treatment.Results showed that all bacterial media treatments increased callus fresh weight and nitrogen concentration of media significantly as compared to that of MS control media. Bacterial media treatment of 2.5 (v/v) gave the highest values of callus fresh weight and nitrogen concentration (518 mg and 3.3089%) respectively, whereas MS control media had the lowest values (193 mg and 0.01%) respectively. There was a significant increase in callus fresh weight at 60 days incubation over that of 30 days incubation. The combination of bacterial media treatment at 2.5 (v/v) and incubation period of 60 days recorded the highest significant increase in callus fresh weight (631 mg), whereas the combination of MS control media treatment and incubation period of 30 days had the lowest value (166 mg) in this respect.
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