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Industrial effluents that carry dyestuff into natural water systems are serious environmental concern. Complex aromatic structures of dyes make them more stable and more difficult to remove from the effluents discharged into water bodies. In the present study, removal of reactive blue 29 dye with chitosan and modified chitosan with Cu complexes from aqueous solution was investigated in a batch adsorption system with respect to the changes in the contact time, pH of solution and chitosan dosage. Adsorption isotherms of the dye onto chitosan were also studied. The results revealed that the adsorption capacity of chitosan with Cu complexes is lower than that of chitosan without Cu complexes. Also effect of H2O2 on adsorption when we used chitosan without Cu complexes is more considerable. The results also demonstrated that adsorption capacity of reactive blue 29 dye on chitosan was higher at lower pHs. Finally, the Langmuir isotherm showed the best conformity to the equilibrium data.