SARS-Cov 2 was present in some air samples that were collected from patient's rooms.• MD8 airscan sampler showed suitable performance for sampling SARS-like viruses. • PTFE and gelatin filters have high performance for the sampling of the viral particle size. • RT-PCR has been commonly used for the detection of SARS-CoV-2 in air samples.

High-performance liquid chromatography with diode array detection (HPLC-DAD) is used to characterize mixtures from chlorophyll u degradation experiments. Overlapping chromatographic peaks are resolved by means of the heuristic evolving latent projections (HELP) method. The HELP method is a self-modelling curve resolution method. No assumptions are made about spectral and/or chromatographic peak shape. In the first step the method establishes the real noise level in the data by use of the so-called zero-component regions. This information is used to reveal selective chromatographic information and the number of chemical species at every retention time in unresolved chromatographic peaks. Utilising the selective chromatographic regions in combination with the zero-concentration windows, unique resolution into concentration profiles and spectra of the pure chemical species is accomplished. HPLC-DAD data from six chlorophyll u degradation experiments were analysed. Consistent results were obtained even with very similar spectra for six or seven overlapping chemical components. KEY WORDS

Introduction: Many patients in coronary care unit (CCU) suffer from decreased sleep quality caused by environmental and mental factors. This study compared the efficacy of foot reflexology massage, foot bath, and a combination of them on the quality of sleep of patients with acute coronary syndrome (ACS). Methods: This quasi-experimental study was implemented on ACS patients in Iran. Random sampling was used to divide the patients into four groups of 35 subjects. The groups were foot reflexology massage, foot bath, a combination of the two and the control group. Sleep quality was measured using the Veran Snyder-Halpern questionnaire. Data were analyzed by SPSS version 13. Results: The mean age of the four groups was 61.22 (11.67) years. The mean sleep disturbance in intervention groups (foot reflexology massage and foot bath groups) during the second and third nights was significantly less than before intervention. The results also showed a greater reduction in sleep disturbance in the combined group than in the other groups when compared to the control group. Conclusion: It can be concluded that the intervention of foot bath and massage are effective in reducing sleep disorders and there was a synergistic effect when used in combination. This complementary care method can be recommended to be implemented by CCU nurses.

Ammonia nitrogen compounds in the wastewaters and effluents have harmful effects on water resources. Ion exchange with zeolites is a separation process for ammonia removal from effluents. The objective of this research was to study the efficiency of an ammonia removal and the factor affecting to this process. The Clinoptilolite was obtained from Semnan mines at the north part of Iran. The samples were grounded and sieved based on the U.S. standard mesh number 20, 30, 40 and conditioned by ammonia sulfate and sodium chloride solutions. The characteristics of samples for ammonia removal and the selectivity sequence for adsorbing interfering cations were then determined. Results shown that the average ion exchange capacity of zeolite in batch and continuous systems were 6.65-16 and 16.31-19.5 mg NH 4 + /g zeolite weight, respectively. In study on the zeolite for selective cations showed the ranking of K + , NH 4 + , Na + , Ca 2+ and Mg 2+ respectively. Results indicated that high level of regeneration (95-98%) might be achieved with NaCl solution. Based on the results, Clinoptilolite may be effective applied in wastewater treatment, both from technical and economical aspects.

–The products of chlorophyll allomerization in methanol were isolated and analyzed by open column sucrose chromatography, liquid chromatography mass spectrometry (LCMS) and DAD‐HPLC (diode‐array high‐performance liquid chromatography). Four main bands were found with molecular ions of (a) 908, (b) 938, (c) 938 and (d) 938, consistent with the structures (a) 132‐hydroxy‐chlorophyll a (II), (b) and (c) Mg(II)‐31,32‐didehydro‐151‐hydroxy‐151‐methoxy‐rhodochlorin‐15 acetic acid δ‐lactone 152‐methyl 173‐phytyl ester and its epimer (III) and (d) Mg(II)‐31,32‐didehydro‐rhodochlorin‐15‐glyoxylic acid 131,152‐dimethyl 173‐phytyl ester (IV), evidence enhanced by UV/visible spectroscopy, chromatographic coelutions and chemometrics. Chlorophyll a was degraded both in the dark and light, under O2 and N2. DAD‐HPLC of the resultant degradation mixtures were analyzed using the chemometric heuristic‐evolving latent projection method for resolution. Ultraviolet/visible spectra of II and III are reproducibly extracted from the mixtures after a short degradation time, whereas III and IV are the dominant compounds after longer degradation times. Changes in relative elution order of IV using open column chromatography and reverse‐phase HPLC are established. A possible allomerization pathway is proposed.