Background: According to Annual Report Iraqi Cancer Registry, prostate cancer were classified within top ten cancers in Iraqi male and composed 6.5 % of all cancer cases. Worldwide studies have shown relationship between LAPTM4B gene polymorphism and risk of prostate cancer. Aim: To study the association of LAPTM4B gene polymorphism with prostate cancer in Iraqi population. Methods: This case control study consisted of 80 prostate cancer patients and 80 healthy individuals as control group. Genotyping of LAPTM4B gene polymorphism is carried out by PCR. DNA was extracted from whole blood and genotyping was achieved with specific primers to amplify gene fragments followed by electrophoresis on agarose gel. Various statistical analyses were applied to analyze the data. Results: The LAPTM4B gene polymorphism was associated with decreased risk of Pca in codominant (OR= 49 %, CI = 0.24 _0.99, P = 0.049, *1/2 versus *1/1) and dominant (OR = 47 %, CI = 0.24 _0.90, P = 0.024, *1/2+*2/2 versus *1/1) inheritance models. Conclusion: Our findings suggest that The LAPTM4B*2 allele significantly decreased the risk of Pca compared to LAPTM4B*1 and consider as protective factor in Iraqi population.