In present paper the concept of WN-semiprime submodule of unitary left R-module  over a commutative ring  with identity are introduced and studied, as a generalization of weakly semiprime submodules. Numerous basic properties of this concept are investigate. Where a proper submodule A of an R-module  is said to be WN-semiprime, if whenever , for , implies that . Furthermore many characterizations of WN-semiprime submodule are introduced. Moreover, some properties of WN-semiprime submodules in some classes of modules are given.