Citation: Ahmed SI, Ali TO, Elsheikh AS, Attia GA, Abdalla AM, et al. (2017) AbstractThere are great concerns regarding the use of synthetic progesterone during breastfeeding due to probable negative effects on future fecundity of male infants. Therefore the present study was conducted to evaluate the effects of exposing male rat pups to depot medroxyprogesterone acetate (DMPA) during lactational period on pubertal testicular histology, morphometry and cells quantitation. Twenty male Wistar rat pups reared to dams treated with DMPA (10 mg/ kg BW) every other day during their early lactation period; were employed to achieve the objectives of this study. Other 20 male rat pups reared to untreated dams served as control. The pups were allowed to reach 90 days old, sacrificed, their testes were dissected and weighed and histological sections were prepared.The results showed that exposing male rat pups to DMPA during the lactational period significantly (P<0.001) affected their testicular histology, morphometry and the quantities of testicular cells. The thickness of the germinal epithelium (GE) and the diameter of the seminiferous tubules (ST) were reduced; while the interstitial space (IS) thicknesses were increased. The testicular cells of the rats reared on dams treated with DMPA experienced varying degrees of apoptosis and count reduction. The ST appeared with unusual configuration with detached and/or folded basal lamina, it has few germinal layers, decreased Sertoli cells (SC) and their lumen contained cells debris and very few sperms; while the wide IS contained few Leydig cells (LC). Exposure to DMPA during the lactational period adversely affects the testicular structure. Thus foretells a negative impact on future fertility. Materials and Methods MaterialsExperimental animals: Ten weeks old 8 pregnant female Wistar albino rats were grouped into two groups (4 rats each) and kept separate away from any stress in sterilized polypropylene cages (90 cm × 45 cm × 15 cm) lined with woody husk. They were kept at 28 ± 7°C temperature in light/dark cycle (12:12 h), fed on commercial pellet and offered water ad libitum. After delivery 4 dams with 20 male pups (Group I) were injected with a placebo to serve as controls; while 4 dams with 20 male pups (Group II) were subcutaneously injected with 10 mg/kg BW of depot medroxy-progesterone acetate (DMPA; Depo-Provera®) every other day (on the first fifteen days) of lactational period. The dam body weights were taken on each day of injection to adjust the dose. The 40 male rat pups were allowed to grow for 90 days where they reach maturity [2,4,[10][11][12].Study design: This one factorial experiment study was designed to investigate the effects of lactational exposure to DMPA on suckling male rat's pups' testis histological structure, morphometry, cells quantities at Citation: Ahmed SI, Ali TO, Elsheikh AS, Attia GA, Abdalla AM, et al. (2017) Testicular Changes in Male Albino Rat Pups Exposed to MedroxyProgesterone Acetate during Lactational Period. J Steroids Horm Sc...