The study was conducted on 400  suspected patients and twenty-four healthy persons, who visited the laboratory of AL-Hakeem hospital and AL-Zahra maternity and pediatrics in AL-Najaf province from October till May 2018.  This study was designed to determine the effects of Giardia lamblia infection on some biomarkers such as (mucin-2 protein (MUC2), ghrelin (GHRL), and obestatin (OB).whereas the numbers and percentage of the infected patient were 48 (12%) vary with different gender;28 (7%) male and 20 (5%), The result of the study revealed that concentration of (MUC2),(GHRL) and (OB) in male and female patients infected with G. lamblia significantly increased (P<0.05) is compared to the control group. Also, it revealed a significant increase (P in concentrations in the total number of patients infected with G. lamblia and the control group. The current study has concluded that the infection with G .lamblia affects some immunological and physiological markers of humans represented by (mucin-2 protein (MUC2), ghrelin (GHRL), and obestatin (OB) as good biomarkers which may be used in the detection of G .lamblia parasite.