| The aim of this study was to investigate the effects of ethanolic extract of 70% Hibiscus sabdariffa (HS) on some physiological parameters and the alteration in enzymatic and non-enzymatic antioxidants in female rabbits. Ten female adult rabbits were divided into two groups (5 rabbits/group), group1: Control group received orally normal saline for four weeks, group2: the treated group received (200 mg/Kg) B.W of the ethanolic extract of HS. Blood samples were collected from animals by cardiac puncture technique. At the end of experiment the results showed that there were significant increase in red blood cell count (RBCC), hemoglobin concentration (Hb) with significant decrease in platelet count (Plt) and non-significant increase in the white blood cell count (WBCC), lymphocyte, monocyte and significant increase in granulocyte count in animals treated by HS extract as compared with control group. The results of liver enzymes showed that there were non-significant increase in activity of serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), and non-significant decrease in serum alanine aminotransferase (ALT) in HS extract treated group as compared with the control group. The results of kidney function parameters explained that there were non-significant increase in level of urea but there were significant (p < 0.05) decrease in level of uric acid in animals treated with HS as compared with control. Moreover, the results of this study showed that there were significant (p < 0.05) decrease in serum triacylglycerol (TAG), cholesterol, superoxide dismutase (SOD), glutathione peroxidase (GPx), but there was non-significant (p > 0.05) changes in catalase activity (CAT), malondialdehyde (MDA), glutathione (GSH), vitamin E (Vit E), and vitamin C (Vit C). The results obtained from this study explained that 70% ethanolic extract of HS increase (RBCC) count, enhance immunity by increasing granulocyte count, and had hypolipidemic and antioxidant effect in female rabbit. Keywords