A series of experiments were conducted to evaluate the toxicity of lemon peel extracts incorporated into mediterranean fruit fly Ceratitis capitata diet. Extracts were obtained with different solvents: diethyl ether, ethyl acetate, and methanol. All three extracts were toxic to some extent; the diethyl ether extract was selected for further studies. Ether extracts of lemon peel were prepared weekly over a 2-month period, from fruits collected on the 1st d of the bioassay. Weekly GC-MS and UV analyses of the extracts demonstrated that the concentration of citral and coumarins decreased in the peel after harvest. We conducted a series of bioassays to evaluate the toxicity of the ether extract, and mixtures of this extract with citral, 5,7-dimethoxycoumarin. and linalool incorporated to C. capitata larvae's natural diet (lemon slices endocarp) at a concentration of 250 microg/g of diet. Significant larvicidal activity can be obtained from a fresh lemon peel extract; however, when the extract was obtained from stored lemons, toxicity decreased. Addition of small amounts of citral or 5,7-dimethoxycoumarin, and linalool to the stored lemon peel extract would bring back the toxicity to the rates of fresh lemons extracts. Finally, female adults of C. capitata fed on diets containing additional amounts of ether extract, 5,7-dimethoxycoumarin, and linalool, were exposed to different photoperiods to test for phototoxicity. The treatment was toxic and affected the oviposition capacity of females depending on photoperiod.
Two new bicyclic phloroglucinols were isolated from the diethyl ether extract of an Argentine collection of the fern Elaphoglossum piloselloides. Structures were established by analysis of their spectroscopic data ((1)H NMR, HRMS, IR) and chemical derivatization. Both compounds show an acute molluscicidal activity against the schistosomiasis vector snail Biomphalaria peregrina.
Four fusicoccane-type diterpenoids (1-4), including the new 1 and 2; four pinguisane-type sesquiterpenoids (5-8); and two aromadendrane-type sesquiterpenoids (9 and 10) were isolated from an Argentine collection of the endemic liverwort Porella chilensis. The biofilm formation of the human pathogen Pseudomonas aeruginosa was inhibited by compounds 3 (53 and 47%), 9 (45 and 41%), and 10 (48 and 37%) at 50 and 5 μg/mL, respectively. Compounds 3, 9, and 10 also produced a slight decrease in bacterial growth and interfere with the process of quorum sensing at the same doses.
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