The transcriptional coactivator Sub1 is a functional component of the PIC implicated in several steps of mRNA metabolism. Sub1 directly and specifically influences the transcription elongation rate by an interaction with the elongation factor Spt5. The results provide a novel mechanistic insight into the process of elongation by RNAPII.

Cohesin is a regulator of genome architecture with roles in sister chromatid cohesion and chromosome compaction. The recruitment and mobility of cohesin complexes on DNA is restricted by nucleosomes. Here we show that the role of cohesin in chromosome organisation requires the histone chaperone FACT in S. cerevisiae. We find that FACT interacts directly with cohesin, and is dynamically required for its localization on chromatin. Depletion of FACT in Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: