Both intestinal mucus and SIgA were important in limiting C. difficile-associated disease severity in this model. A synergistic effect of mucus and IgA was also noted and may be due to protection of SIgA from proteolytic cleavage.

PC supplementation was effective in improving intestinal barrier defense against C. difficile toxin A challenge. PC administration may be a useful therapeutic adjunct in severe cases of C. difficile colitis or in patients who do not improve with conventional treatment.

285menbs were made of energy reflected by the epithelium inside the cheek, it may still be assumed that about one-third of the energy entering the cheek passed through.These studies indicate that living tissue is generally impermeable to all but a narrow b a d of ordinary radiant energy lying between wave lengths of 0.6 to 1.6 micron. Maximum penetration seems to occur at a wave length of 1.15 micron. What physiological or therapeutic effects such radiant energy has is still largely undetermined. A lamp specially designed to deliver rays in the range indicated has been ma!d$e and is available for study.A number of workers recently have demonstrated that it is possible to produce variants among both plants and animals by exposure to ithe effect of X-rays We, in turn!, have endeavored to demonstrate change by a similar procedure applied to the Bacteriophage and to the organism susceptible to the Bacteriophage, following suggestions made by Dr. Olson.Seven strains of B. coli bacteriophage were seleclted and for each of these wecultured the particular strain of B. coli acted upon by its respective strain of bacteriophage. There were selected 7 coli bacteriophages and 6 strains of B. coli for $this series of experiments.These bacteriophages were effective as foliows upon the bacterial strains: one for all the strains, one for 5, one for 2 and 3 could lyse only one strain each of the organisms. There were thus 42 combinations possible in such a set up of tubes in case m y variation were to appear.The organisms were young cultures in the phase of positive logarithmic growth in beef infusion broth and the phages were recent filtrates in the same medium. These were contained within lead free glass test tubes. Exposure to X-ray was carried out at a dlistance of approximately fourteen inches from the target of a Roentgen tube actuated by approximately 75,000 volts of electricity. The exposure period was 30 minutes. Following this treatment each bacterio-The following methods for exposure were followed.at PENNSYLVANIA STATE UNIV on June 25, 2015 ebm.sagepub.com Downloaded from

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