Currently, special attention is paid to the use of industrial by-products, in particular those obtained in huge quantities in the brewing and wine making process, as a source for the production of natural preparations with high biological value. In this study are presented results related to the recovery of beer yeast biomass from the sediments of the beer industry, by obtaining lipid extracts with valuable biochemical composition. Thus, applying the optimized autolysis method, with the use of sodium phosphate buffer at 45°C for the destruction of the cell wall and fractional extraction with hydric, alkaline and acidic solutions, various liquid and solid fractions were obtained from the biomass of brewer’s yeast with varied lipid content. So, in order to optimize the waste processing process of the beer industry and the complex recovery of yeast biomass, it is reasonable to include the lipid fraction extraction stage in the technological flow, after obtaining protein and mannoprotein extracts, which will be useful and as an additional step of purification of the β-glucan fraction.

In this study, the procedure of obtaining new biologically active mannoprotein preparation based on the yeast biomass from the residues of the wine industry, namely the production of Merlot dry red wine is presented. The process for obtaining of the preparation includes lysis of the yeast cell wall with sodium phosphate buffer, extraction of anthocyanins by treatment with 50% alcohol solution, hydrolysis with 1N NaOH, sedimentation of mannoproteins with ethyl alcohol, centrifugation and dissolution of purified sediment in distilled water, standardization of the preparation obtained up to a concentration of 10 mg/ml. As a result, it has been established that the mannoprotein preparation possesses a valuable biochemical composition characterized by a high content of proteins, carbohydrates, anthocyanins, and a wide range of minerals, macro, microelements, and does not practically contain heavy metals. Due to its biochemical composition, the preparation, also, possessed antioxidant activities and high catalase and superoxide dismutase activity. In conclusion, we can mention that the varied biochemical composition, antioxidant, and enzymatic activity highlight the high biological value of the mannoprotein preparation and the enormous potential for implementation in various fields, especially in animal husbandry and viticulture, food and cosmetic industry.

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The properties of nanoparticles have been used in a wide range of potential applications in food industry, medicine, microbial biotechnology, cosmetics, environmental production. Research results of evaluation of the effect of Fe3O4 and TiO2 nanoparticles applicated in large concentration limits on some parameters of pigmented yeast strain Rhodotorula gracilis CNMN-Y-30 are presented in this paper. It was established that nanoparticles selected for study caused toxic effects on β-caroten accumulation and activity of antioxidant enzyme catalase depending on concentration and nanostructure. The strong correlation between concentration of nanoparticles and evaluated components has been revealed at studied yeast strain. Coefficients of correlation varied in limits R² = 0.623... 0.951. For the first time, the determination of β-carotene accumulation and catalase activity rate as functional tests for estimation of toxicity of nanoparticles for pigmented yeast strain Rhodotorula gracilis was effectuated. Thus, it was established that adaptive response of yeast strain to the presence of Fe3O4 and TiO2 nanoparticles has been manifested by modification of the processes of β-carotene biosythesis and catalase activity.