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The aim of this study was to monitor the water quality of Luiz Rau stream, through physicochemical parameters, environmental characteristics of the site, and genotoxicity test in Allium cepa. Four water samples from Luiz Rau stream were collected once a week for one month and some physicochemical parameters were verified. The environmental characteristics were performed by the Quick Assessment Protocol (QAP) and evaluated at the sampling site. For the genotoxicity assay, the Allium cepa test was utilized. In relation to the water physicochemical parameters, low levels of dissolved oxygen (DO) and high levels of ammonia, and phosphorus were obtained, which demonstrates the poor quality of the stream. The QAP results identified the stream as impacted. The mitotic index parameter was altered in the seed exposed to raw water samples in the third and fourth sample collection, demonstrating cell cycle reduction and proliferation respectively; witch can be associated with the presence of contaminated effluents in the water. In relation to the genotoxicity, the micronuclei were increased in seeds exposed to four raw samples in comparison to the control group. Therefore, the results obtained can characterize the Luiz Rau stream as impacted, mostly by anthropic actions, corroborating with other publications.

SUMMARY The interaction established between man and their pets has been increasing progressively. The diagnosis of animals carrying methicillin-resistant Staphylococcus pseudintermedius (MRSP) becomes important since positive results for this opportunistic bacteria has been found in humans. The aim of this study was to investigate methicillin-resistant Staphylococcus pseudintermedius (MRSP) in domestic dogs attended in a veterinary clinic and shelter dogs. Anal and nasal swabs from 53 domestic dogs and 46 shelter dogs were collected between the months of August and October of 2015, resulting in 198 samples, which were sent for routine laboratory analysis and subsequently used for this study. Phenotypic tests for bacteria identification and susceptibility to oxacillin were performed for the investigation of MRSP. A total of 23 samples (11.6%) from 21 dogs were positive for S. pseudintermedius, however none of the isolates were MRSP. Nasal and anal swabs had similar positivity and two dogs were carriers of the bacteria in both analyzed sites (anal and nasal). Our results showed a higher positivity for S. pseudintermedius in domestic dogs compared to shelter dogs, as well as indicating the importance of collecting more than one colonization site. This is the first study to perform a comparison among different animal creating sites in Brazil.

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