Purpose: Fungal infections are one of the most common skin infections. Athletes, especially in contact sports such as wrestlers are the group at risk of fungal skin infections (FSI). The aim of this study was to determine prevalence of FSI and some effective factors in wrestlers in Tehran, Iran. Methods:This study was a cross-sectional descriptive study which was conducted in 2009 and subjects were wrestlers of wrestling clubs of Tehran, Iran. In this study the prevalence of FSI and its related factors was collected based on clinical history, physical examination and laboratory tests. We also determined the rate of dermatophytic contamination of wrestling mats. The relationship between independent variables and incidence of fungal infection analyzed by Chi square test and regression analysis. Results:In this study, out of 454 wrestlers, 111 (24.5%) subjects had suspicious fungal skin lesions on physical examination. The Prevalence of FSI was 8.2% in all of the wrestlers (34.2% of the wrestlers with suspicious lesion). Malassezia furfur (50%) and trichophyton tonsurans (30%) were the most common causative agents. Epidermophyton floccosum (7.5%), Trichophyton rubrum (5%), Candida albicans (5%) and Trichophyton mentagrophytes (2.5%) were other isolated fungi. Eleven wrestling mats (44%) were contaminated with different fungal organisms. Conclusion:The prevalence of fungal skin infection in Iranian wrestlers is relatively high. Appropriate preventive measures need to be prepared and implemented to reduce incidence of FSI in wrestlers.