Thaumetopoea pityocampa, the winter pine processionary moth, is one of the most important pests of pine trees in the Mediterranean region. To learn more about the refugial areas within the greater refugium of Greece, samples from 15 local populations were collected. Analysis of the Cytochrome Oxidase I region identified 15 haplotypes. One strongly supported clade was found, separating the Aegean island of Lesvos from the mainland populations. Mdiv analysis showed that this clade diverged from the general clade 155,000 y.a., suggesting the sea level changes during the Quaternary Period as a possible reason for its seclusion. Additionally, comparisons with similar studies in this region revealed a possible colonization of the eastern Aegean islands from the Turkish mainland rather than from Greece. However, no strong isolation-by-distance events were detected among the mainland populations, which could be attributed to the joint effect of regular gene flow and the lack of insurmountable geographic barriers. Finally, regarding population structure, Bayesian analysis as well as neutrality tests pointed towards an ongoing population expansion which verifies the potential invasiveness of this pest species, something that will have to be dealt with under the influence of climate change.

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In the context of plants or plant products protection by harmful organisms, measures have been taken by EU countries in order to prevent their introduction and establishment into the EU, and also limit their expansion in case they do enter. Such a case is Bursaphelenchus xylophilus (Parasitaphelenchidae, Nematoda), already recorded in Portugal and Spain. So, Member States should take all the appropriate steps in order to monitor and confine if necessary susceptible plants and/or plant products. Such measures include annual surveys even in countries where pine wilt disease does not occur yet. Therefore, national survey programs are widely established, sampling and examining samples from pine trees showing suspicious symptoms that could potentially be attributed to B. xylophilus. In this direction, such a network has also been established in Greece collecting and examining wood samples nationwide. In total, 123 wood samples were collected from conifer trees of Northern and Central Greece. Though B. xylophilus was absent from all samples examined, four other Bursaphelenchus species were identified. In addition, other nematode taxa were also recorded, including several phytophagous, microbivorous as well as predatory nematode species. This highlights the fact that besides preventing the introduction of B. xylophilus in Greece, national survey programs can significantly contribute to and enhance our knowledge of the indigenous nematode species.

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