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The mechanisms by which some crustaceans permanently or temporarily maintain their body fluids at osmotic or ionic concentrations different from those of their environment include the active transport of ions across the gills and other surfaces (

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The cardiac ganglion from Homarus americanus was investigated for the purpose of providing biochemical and histochemical information as to the identity of the neurotransmitter(s) utilized by this system. Three techniques were employed in this study: (1) the glyoxylic acid histofluorescence staining technique (GA), which showed fluorescence characteristic of catecholamines localized in the five motor neurons; (2) high-voltage electrophoresis (HVE) in one dimension followed by ascending chromatography in the second dimension, which indicated incorporation of label from tritiated tyrosine into norepinephrine (NE) and small amounts of dopamine (DA); (3) high-pressure liquid chromatography with electrochemical detection (HPLC/EC), which indicated the presence of endogenous norepinephrine.