The current study was performed to determine the kinds of organophosphorus pesticides residue in milk sample in state of Khartoum-Sudan. 66 samples were collected from breeding farms widespread the state, regarding the areas of high farms concentration. Samples were collected in sterile vacutainers frigid, extracted and analyzed using gas chromatograph GC/FID. Two pesticides belong to organophosphorus group were detected (Dimethoate and Chloropyrifos). 4% of Khartoum bahri samples contained the two pesticides, while Khartoum and Omdurman recorded 21% and 5.26% respectively. 92% of dimethoate positive samples from Khartoum Bahri area exceeded the maximum residue limits, while Khartoum and Omdurman showed the same percentage 84.2%. All Choloropyrifos positive samples were below the maximum residue limits.