Summary. Selected 1,10-phenanthroline hydrochlorides, quaternary salts and metal chelates are bactericidal to Staphylococcus aureus. Streptococcus pyogenes (Group A), Clostridium perfringens, Escherichia coli and Proteus vulgaris in serum broth in that general order of decreasing sensitivity; certain hydrochlorides and metal chelates are also bactericidal to Staphylococcus aureus in distilled water in the absence of added metal ion.Metal chelates with similar or identical bactericidal titres following 48 hours' incubation with Staphylococcus aureus. Streptococcus pyogenes (Group A) or Escherichia coli differ greatly in their rate of kill. Generally, the labile Gu(II), Gd(II), Zn(II) and Mn(II) phenanthroline chelates are rapidly lethal to these bacteria while the corresponding more inert Fe(II), Ni(II) and Ru(II) compounds are more slowly bactericidal; the Go(II) analogues are the most slowly lethal, probably owing to the formation of highly inert cobalt (III) species. These results suggest that the kinetic reactivity of the metal chelate is more important than its thermodynamic stability in governing rate of bactericidal action. Other important factors are the lipophilia of the active species, which may be the phenanthroline ligand or a metal-containing derivative, and the permeability of the organism to it.Gomparison of the bactericidal activity and rate of kill of Staphylococcus aureus and Escherichia coli by selected metal chelates and by a series of unrelated but clinically-useful antibacterials suggests that the former compounds warrant investigation for the sterilization of skin surfaces both prophylactieally and therapeutically. INTRODUCTION.The bacteriostatic activities of a series of metal chelates, quaternary salts and base hydrochlorides derived from 1,10-phenanthroline and 2,2'-bipyridine bases against a number of typical Gram-positive and Gram-negative bacteria and Mycobacterium tuberculosis have been reported by Dwyer, Reid, Shulman, Laycock and Dixson (1969). The present paper reports the bactericidal activities of selected phenanthroline metal chelates and related substances against a similar series of Gram-positive and Gram-negative bacteria and indicates that speed of chelate bactericidal action depends on the nature of both the metal ion and the ligand, that is, on the properties of the metal chelate as a whole. The bactericidal

No abstract

The results of this randomized double blind, placebo controlled trial of a single oral dose of 2 g tinidazole or placebo approximately 12 hours before hysterectomy showed the incidence of infective morbidity in patients who received placebo was 12.2% compared with 1.9% in patients who received tinidazole, (p = 0.045). The mean serum concentration of tinidazole at the time of surgery was 35 mcg/ml, which was well in excess of the minimum concentration required to inhibit all of the 95 anaerobes isolated in this study. Serum tinidazole concentration remained adequately inhibitory for at least 24 hours after surgery. The ability of tinidazole prophylaxis to reduce further our already low rate of febrile morbidity was considered a worthwhile gain. It is now Unit policy to give this agent prophylactically to all patients if the integrity of the vagina is likely to be breached at the time of surgery.

No abstract