Background: Cervical cancer caused by human papilloma virus (HPV), is the second most common cancer for women. This cancer is distributed worldwide, with ~80% of cases are found in the developing countries. In Indonesia, data of HPV genotypes are still limited and do not represent all regions of the country. Thus, here we report genotyping of HPV samples collected from the Dr. Soetomo Hospital Surabaya Indonesia patients, in 2013. Materials and Method: A cross sectional study was performed using 68 paraffin blocks of low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) cervix.

Objective: The objective of this study was to discover the possible correlation between p16INK4A expression and the LR/HR-HPV infection in condyloma acuminate (CA) lesions. Materials and Method: This cross-sectional study was conducted during January-December 2017 on 33 CA patients. The expression of p16 INK4A was detected by immunohistochemistry (IHC) staining. The positive interpretation was carried out by scoring which score 0 was negative, score 1 was sporadic, score 2 was focal, and score 3 was diffuses. The HPV genotypes were identified by reverse line blot, and 40 genotypes of HPV detected, including HR-HPV (HPVs 16, 18, 26, 31, 33,35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68a, 68b, 69, 73, and 82) and LR-HPV (HPVs 6, 11, 40, 42, 43, 44, 54, 55, 61, 62, 64, 70, 71, 72, 81, 83, 84, 87, 89, and 90). Results: The expression of p16 INK4A was significantly correlated with HR-HPV infection. Patients infected with HR-HPV had 0.644 times higher possibility to express p16 INK4A gene compared to those infected with LR-HPV. LR-HPV genotypes detected in CA patients were HPVs 6, 11, 42, 61, 54, 81, 87, 89, and 90 and HR-HPV genotypes were HPVs 18, 26, 45, 51, 52, 67, 68B, 69, and 82. LR-HPV was found in 19/33 of patients and HR-HPV was in 14/33 of patients. The expression of p16 INK4A in CA lesions was diffuse in15.2% of patients, was focal in 24.2% of patients , was sporadic in 39.4% of patients were, and was negative in 21.2% of patients . In LR-HPV group, there was no diffuse expression, focal expression was observed in 15.8%, sporadic in 47.4%, and negative in 36.8%, while in HR-HPV group, p16 INK4A expression was detected in all lesions , in a way that its expression was diffuse in 35.7%, focal in 35.7%, and sporadic in 28.6%. Conclusion: IHC is a routine method in histopathological diagnosis, therefore the detection of p16 INK4A expression by IHC can be used as a biomarker for HR-HPV infection diagnosis.

ABSTRAK Objectives: To report one malignant Brenner tumor (MBT) case in Dr. Soetomo Hospital, Surabaya, Indonesia, that underwent therapy from August 2016 to August 2017. Case report: A woman aged 62 years underwent total abdominal hysterectomy and bilateral salphingo-oophorectomy surgery with histopathologic results of malignant Brenner Tumor. From immunohistochemical examination the expression of p63 was positive. The patient had been treated with Paclitaxel carboplatin chemotherapy for 5-cycles, with the results of the last postchemotherapy evaluation showing no recidive mass. Conclusion: There was only one case of malignant ovarian Brenner Tumor in Dr Soetomo Hospital, Surabaya, Indonesia, in 2014-2016. The diagnosis can only be based on anatomic pathology examination. The specific tumor markers for malignant Brenner tumors have so far not been found. Keywords: Malignant Brenner Tumor; epithelial ovarian tumor; p63. Tujuan: Melaporkan satu kasus pasien dengan tumor Brenner di RSUD Dr. Soetomo, Surabaya, Indonesia, yang menjalani terapi dari bulan Agustus 2016 hingga Agustus 2017. Laporan kasus: Seorang wanita berusia 62 tahun menjalani histerektomi abdominal total dan operasi salphingo-ooforektomi bilateral dengan hasil histopatologis Tumor Brenner ganas. Dari pemeriksaan imunohistokimia, ekspresi p63 didapatkan positif. Pasien telah dirawat dengan kemoterapi Paclitaxel carboplatin selama 5 siklus, dengan hasil evaluasi post-kemoterapi terakhir tidak menunjukkan massa residif. Simpulan: Hanya terdapat satu kasus Tumor Brenner ovarium ganas di RSUD Dr Soetomo, Surabaya, Indonesia, antara 2014-2016. Diagnosis hanya dapat didasarkan pada pemeriksaan patologi anatomi. Penanda tumor spesifik untuk tumor Brenner ganas sejauh ini belum ditemukan. Kata kunci: Tumor Brenner ganas; tumor epitel ovarium; p63

Tujuan: Penelitian ini bertujuan untuk mengetahui prevalensi lesi prekanker serviks dengan cara melakukan skrining kanker serviks menggunakan pemeriksaan Pap smear.Bahan dan Metode: Penelitian ini merupakan penelitian observasional deskriptif dengan metode pendekatan cross sectional yang dilakukan pada 140 wanita, terdiri dari 90 orang dari Puskes-mas Tanah Kali Kedinding Surabaya, dan 50 orang dari Rumah Sakit Mawadah Mojokerto, dengan usia 20-70 tahun. Pemeriksaan spesimen sitologi serviks dengan pengecatan Papaniculaou dan klasifikasi hasil pemeriksaaan sitologi berdasar-kan klasifikasi Papaniculaou dan sistim Bethesda. Pemeriksaan inspeksi visual asam asetat (IVA) adalah metode lain untuk skrining kanker serviks dilakukan dengan mengoleskan asam asetat 5% pada area serviks dan melakukan pengamatan satu menit kemudian.Hasil: Hasil pemeriksaaan Pap smear menunjukkan Papaniculaou kelas I (sama dengan normal pada klasifikasi sistim Bethesda) yaitu 12.1%, kelas II (sama dengan NILM pada klasifikasi sistim Bethesda) yaitu 86, 4%, dan kelas III (sama dengan LSIL pada klasifikasi sistim Bethesda) yaitu 1,4%. Hasil pemeriksaan IVA menunjukkan 6,43% positif dan 93,57% negatif. Hasil pemeriksa-an IVA positif terdapat pada 9/140 orang yang merupakan proses keradangan dan infeksi, bukan merupakan lesi prekanker.Simpulan: Prevalensi lesi prekanker yaitu 1,4%. Pemeriksaan IVA menunjukkan hasil positif semu yang disebabkan oleh proses radang atau infeksi pada serviks.

Introduction: Breast cancer and cervical cancer are common cancers in females. Both of these cancers can be detected early. Early detect vion can reduce cancer morbidity and mortality. The community outreach activities are required to increase knowledge about breast cancer and cervical cancer, in addition to screening as preventive efforts for these two cancers.Methods: The activity of raising knowledge about the prevention and early detection of breast cancer and cervical cancer was carried out through community counseling to 94 females who were members of the PKK and young females in the Kecamatan Proppo, Kabupaten Pamekasan. Pre and post tests were given before and after the community counseling. After the community counseling, 86 participants were screened for breast cancer by clinical palpation and 46 participants were screened for cervical cancer using the pap smear method.Results: The result of the post test showed a 27.53% increase in knowledge. The results of the pap smear examination on participants showed 96% concluded as Papanicolau Class II, 2% Class I, and 2% Class IV. The patient with class IV results was referred to an obstetrician.Conclusion: Community counseling about breast self examination (BSE) and Pap smear can increase people's knowledge about early detection of breast cancer and cervical cancer.