BACKGROUND: The ratio change of type I and type III collagen in the peritoneal tissue can be associated with defects in collagen synthesis caused by the extracellular matrix’s degradation. Matrix metalloproteinase-2 (MMP-2) is an enzyme that contributes primarily to the degradation of this extra cell. AIM: This study aimed to analyze the differences in expression of COL1A1, COL3A1, and MMP-2 mRNA and the relationship between these expressions in adult inguinal hernias and the expression ratio between the COL1A1/COL3A1 genes. METHODS: This study was an observational study with a cross-sectional comparative study design, where the sample was adult inguinal hernia patients who were taken from the aponeurosis tissue m. external obliquus performed at the time of surgery, while control was a non-herniated patient. The sample RNA was isolated, followed by cDNA synthesis, and examined by real-time polymerase chain reaction. RESULTS: The mean values of expression for COL1A1, COL3A1, and MMP-2 in the case group were 40.02 ± 181.38 copy number, 33.70 ± 143.62 copy number, and 31.78 ± 84.47 copy number. Meanwhile, the expression values for COL1A1, COL3A1, and MMP-2 in the control group were 40.247 ± 162.837 copy number, 13.35 ± 37.43 copy number, and 20.58 ± 48.95 copy number. CONCLUSIONS: Our study showed a difference in COL3A1 expression between the hernia and non-hernia groups, and no difference was found in the expression of COL1A1 and MMP2 between the hernia and non-hernia groups.

Introduction. Peritonitis has a high mortality rate, SOFA is a scoring system that represents 6 organ functions namely respiration, blood coagulation, liver, cardiovascular, Central Nervous System, and Kidney the higher the SOFA score the higher the possibility of mortality, qSOFA is a new scoring , practical and has a strong predictive value. Method. This study was a diagnostic study in the form of a suitability test to assess the sensitivity, specificity, PPV and NPV, cut of points and the suitability of the SOFA and qSOFA scoring systems in predicting outcomes of patients with secondary peritonitis in RSMH Palembang. Results. Data processing was performed on 27 study subjects during November 2017-June 2018. SOFA prognosis test results with a cut-off point >5 obtained a sensitivity of 84.2%, 100% specificity, PPV 73.68%, NPV 100%, prognosis test results qSOFA with a cut off point> 2 Obtained a sensitivity of 91.7%, specificity of 100%, PPV 91.66%, NPV 80%, Conclusion.The qSOFA scoring system was more efficient than SOFA in predicting mortality