Giardiasis is one of commonest intestinal protozoa causing diarrheal worldwide especially in daycare centers. The present study aimed to detect prevalence of G. lamblia and its risk factors among children by using stained microscopic smears and immunochromatographic test (ICT). A cross-sectional study was carried out among 100 children (4 to 15 years) suffered from diarrhea at Pediatric Outpatient Clinics, Aswan University Hospitals. Sheet was filled out one each child or his parents as to name, sex, age, and previous history of giardiasis. All samples were examined by ICT, and microscopyThe results showed that the prevalence of giardiasis among children was 49%, & 41% by using stained microscopic examination and rapid ICT (Epituub USA) respectively. Sensitivity and specificity of rapid test were (77.6% & 94.1%) respectively without cross-reactivity with other parasites, with potentially useful rapid test in absence of unqualified lab technician or in epidemiologic surveys. Giardiasis is more prevalent in rural areas children than in urban ones.