No abstract

This study aimed to investigate the epidemiology and the possible ways of control of Pasteurella multocida (P. multocida) microorganism as a genital and a respiratory pathogen in rabbit bucks during the period from 2012-2016. Two hundred semen and nasal samples randomly collected from 23-35-week-old apparently healthy breeding bucks representing 30 rabbit farms located in different districts at Giza Governorate. The examined rabbit bucks suffered from low reproductive activity. Serological identification was performed by using slide agglutination test (SAT), gel-diffusion precipitin test (GDPT) and enzyme linked immunosorbant assay (ELISA). Molecular identification of the isolated strains was performed using polymerase chain reaction (PCR). The correlation between the presences of P. multocida and reproductive disorders in rabbit bucks was investigated by measuring semen parameters (advanced sperm motility %, live spermatozoa %, Sperm concentration x 10 6 / ml and sperm abnormalities %) in alternative husbandry systems. The results revealed that isolation of 7 isolates of P. multocida with an incidence of 3.5 %. The in vitro antibiotic sensitivity test revealed that the isolated strains were highly sensitive to thiamphenicole, gentamycin, ciprofloxacin, amoxycillin, amoxycillin + clavulanic acid, clindamycin, colistin sulphate,