The ichthyofauna in relation to water quality was studied on monthly basis from March, 2010 to February, 2011 in the Kangsabati Reservoir, West Bengal. The study revealed that physicochemical parameters of Kangsabati Reservoir were congenial for 39 fish species of commercial importance, belonging to 7 orders, 15 families, and 26 genera. The Cypriniformes were dominant with 17 species, followed by Siluriformes and Perciformes, with 7 species each, Channiformes with 3 species, Osteoglossiformes and Synbranchiformes with 2 species each, and Anguilliformes with 1 species. Regarding their conservation status, 27 species were of least concern, 1 species was vulnerable, 6 species were near threatened, 1 species was data deficient, and 4 species were not evaluated (IUCN-Version 2014.1). Economical values have also been evaluated. Water parameters such as temperature, pH, alkalinity, dissolved oxygen, hardness, free CO2, salinity, total inorganic nitrogen, and phosphate were recorded and found suitable for fish production. Conductivity, transparency, and high chloride level are minor limiting factor that may needs rectification for improved fisheries management.