Humans need amino acids, especially the essential ones, which are obtained from the proteins ingested in the diet. With the rampant growth of the world population, it is necessary to increase the protein supplies, especially those of plant origin, which now play a major role as a food source. Leaves of ora-pronobis (Pereskia aculeata Miller) are a high quality protein source and have been used in the traditional cuisine of Minas Gerais for decades. However, it is important to analyze the anti-nutritional and/or toxic factors to consider the use of the plant leaves as an alternative source of nutrients. This work aimed to study the presence of proteic anti-nutritional factors, such as protease inhibitors and lectins, and to evaluate the "in vitro" digestibility throughout Tricine SDS-PAGE of these factors extracted from ora-pro-nobis (Pereskia aculeata Miller) leaves. The extraction of protease inhibitors and lectins from ora-pro-nobis leaves was performed in phosphate buffer. The partial purification of the fractions presenting activity of trypsin inhibitor and lectins was performed by ammonium sulfate precipitation and gel-filtration chromatography. The "in vitro" activity of the fractions with protease inhibitor and lectins, with or without heat treatment in the presence of digestive enzymes, showed that the cooking time of 1 minute was sufficient for protease inhibitors degradation. However, this time was not satisfactory for lectins, which remained resistant even after heating. This work shows that with proper cooking, ora-pro-nobis leaves can be used in the diet as a source of aminoacids. RESUMOO homem necessita na sua alimentação de aminoácidos, especialmente os essenciais, os quais são obtidos a partir das proteínas ingeridas na alimentação, que podem ser tanto de origem animal quanto vegetal. Com o crescimento desenfreado da população mundial será necessário multiplicar o suprimento de proteínas, principalmente aquelas de origem vegetal, que passam a desempenhar um papel de grande importância como fonte de alimento. As folhas de ora-pronobis (Pereskia aculeata Miller) são uma fonte protéica de alta qualidade e vem sendo utilizada na culinária mineira há décadas. Porém, para a utilização de folhas vegetais como fonte alternativa de nutrientes, é necessário o conhecimento da presença de fatores antinutricionais e/ou tóxicos que possam afetar o valor nutricional dos alimentos. Esse trabalho teve como objetivo realizar o estudo de fatores antinutricionais, inibidores de protease e lectinas, e avaliar a digestibilidade "in vitro" em SDS-PAGE de Tricina desses fatores extraídos das folhas de ora-pro-nobis (Pereskia aculeata Miller). Foi realizada a extração dos inibidores de protease e lectinas das folhas de ora-pro-nobis em solução tampão fosfato, seguida de precipitação de sulfato de amônia e parcial purificação das frações com atividade de inibidor em tripsina e lectinas, utilizando a coluna de separação por exclusão molecular. A digestibilidade "in vitro" das frações com atividade de inibição de p...

This study aims to highlight the contribution of the Association Engineers Without Borders -Juiz de Fora Nucleus (ESF-JF) for the professional training of students, as well as to prove the social impact of their work for the local society as an extension project linked to the Faculty of Engineering, Federal University of Juiz de Fora. To this end, internal documents of the organization were analyzed, in the period from 2015 -2021, in order to quantify the number of projects developed, social actions, people impacted by projects and members who participated in the Association. Thus, it was found that 86 projects were concluded, resulting in 11108 people impacted. In addition, during the evaluated period, more than 24 social actions were carried out, and in 2020 alone, more than 3 tons of food, 830 cleaning and personal hygiene items and 2300 fabric masks were donated. In the analyzed period, the ESF-JF had 168 effective members, of which 40 students were linked to the extension project "UFJF + Engineers Without Borders: Promoting social development". Therefore, it was possible to ratify the contribution of the institution's work to the development of its members on a personal and professional level, receiving humanistic training and technical knowledge, in addition to meeting the demands of local society, filling the social vacuum left by the State.