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Background: Ritonavir (RTV) is an anti-retroviral drug used in the treatment of HIV/ AIDS. Many times, RTV is used alone and in combination with many anti retroviral drugs. So far, around seventy nine analytical methods have been reported for various studies on analysis of RTV in bulk, pharmaceutical formulations and biological fluids. Aim: To summarize the various analytical techniques such as Chromatog raphy, Spectrophotometry; Capillary electrophoresis and also hyphenated techniques such as LC-MS/MS for estimation of Ritonavir. Method: The present article deals with the comprehensive de tails about the type of various analytical techniques such as Chromatog raphy, Spectrophotometry; Capillary electrophoresis and also hyphenated techniques such as LC-MS/MS, and their applicability in analysis of RTV. These techniques are either explored for the quantification, detection of metabolite and also for stability-studies of the RTV. Conclusion: The present studies re vealed that HPLC technique along with the spectro-scopic have been most widely explored for the analysis. The investigatory review may provide the comprehensive details to the researchers who are working in the area of analytical research of RTV.

Garenoxacin Mesylate (GRN) is a quinolone antibacterial agent for the management of bacterial illness. The established method illustrates a development of simple, specific and robust method for the analysis of GRN using a reversed phase high-performance liquid-chromatography method on PrincetonSPHERE ULTIMA C18 column (250 mm x 4.6 mm, 5 μm) with PDA detection was carried out at 280 nm. A seven-factor eight-run Taguchi design was applied to factor screening studies and central composite design with α = 1 was utilized to optimization of experimental parameters of RP-HPLC for obtaining anticipated chromatographic resolution. Risk assessment, examine was executed to understand the basic method parameters. From the risk assessment three independent parameters such as percent acetonitrile content, mobile phase pH and flow rate were selected and study the impact of these parameters on the responses. From the design information the optimized chromatographic conditions comprises of acetonitrile: water in the ratio of 60:40 % v/v, pH 3.5 of aqueous phase marked using 0.1 % ortho phosphoric acid, separately. The percent recovery study was executed at three levels, was obeyed in the range of 99-101 %. Additional the method was validated as per ICH guidelines.

Review: 23 refs.