Equine herpesvirus-1 is a highly prevalent and frequently pathogenic infection of equines. The most serious clinical signs of disease ranging in severity, from mild respiratory distress to abortion in pregnant mares, neonatal foal death and neuropathogenic disorders. Inactivated EHV-1 adjuvanted vaccines consider the best way for controlling infectious disease. In this research, the prepared EHV-1 adjuvanted vaccines was completely inactivated by binary ethyleneimine (BEI) with (0.008M) and proved to be, sterile, pure, safe and potent when inoculated on VERO cells, mice and horses. Challenge was applied only in inoculated mice to detect the role of all prepared vaccines in the reduction of virus clearance and excretion. There was no any undesirable post vaccinal reaction in horses vaccinated with the prepared vaccine reconistitued in saponin, DEAE-Dextran (100mg/dose) and adjuvanted with Montanide MT Gel-01. The immunogenicity of vaccinated mares adjuvented with different three types of vaccine was assayed by different serological tests revealed that maximum antibodies titre was achieved at 3-4 months' post vaccination and EHV-1 inactivated vaccine reconstituted in DEAE-Dextran (100 mg/dose) is the best one of them followed by the vaccine reconstituted in saponin and vaccine adjuvanted with Montanide MT Gel-01.