Chitin and chitinolytic are beneficial enzymes for their biotechnological applications. Chitinases contribute to the generation of carbon and nitrogen in the ecosystem and can prohibit many fungal ailments that can threaten crop production worldwide. The goal of this work was the production and characterization of chitinase enzymes from bacteria isolated from the western region, Saudi Arabia for biocontrol fungal pathogens. Colloidal chitin from shrimp shells was prepared and used for isolation of chitinolytic bacteria on Mineral chitin agar medium from different sources. The most active isolates were AMM1which was characterized and identified as Alcaligenes aquatilis using 16SrRNA.. In conclusion, purified chitinase was success to produce from Alcaligenes aquatilis for medical uses and biocontrol process.