The aim of this study was to assess the impact of lockdown during COVID-19 pandemic on psychological wellbeing among healthy working adults. This qualitative, cross-sectional study was conducted in Kanyakumari District, Tamilnadu, and the southernmost state of India. A convenient sample of 610 healthy working adults was participated and completed a self-administered questionnaire. The data collection tool contains two sections including baseline information, assessment of psychological wellbeing. Chi-square χ² test was used to test the association between demographic variables and scores of psychological wellbeing. One way ANOVA test was used to compare the mean score between groups. Descriptive statistics were used to describe the frequencies of variables. A significant P value was set at 0.05 at 95% confidence interval. The majority of participants (31.8%) belonged to 41-50 years of age. More than half (56.9%) of participants were females. The study findings revealed that 16.4% were had poor psychological wellbeing, 53.8% were had an average level, 21.3% had a good level and 8.5% were had an excellent level of psychological wellbeing. Additionally, gender, age, monthly income, history of quarantine, tested positive for the Corona Virus had a strong association with Psychological wellbeing. The study results concluded that the lockdown during the COVID-19 pandemic had a strong association with the psychological wellbeing of working adults. It also suggested having highly prioritized actions for early recognition of psychological problems, expanding counseling activities by the health authorities to avoid negative outcomes due to the Covid-19 pandemic lockdown.