tality of HCV strains healthcare systems worldwide, the Direct-acting antivirals (DAAs) give promising prospects to clear HCV infection and reduce HCV disease burden in the soon future (6). Whereas the elimination of hepatitis C virus by 2030 is a global goal for the World Health Organization (WHO). The epidemiology of hepatitis C in Yemen is still not well described, and it needs to define the prevalence in different populations and define the areas of a future epidemiological study in Yemen which are the objectives of this systematic review (7,8). Bacground and Aims:To identify and well-characterize the prevalence of hepatitis C virus infection in different populations in Yemen. Also, to determine the future epidemiological study areas in Yemen. Materials and Methods: A systematic review was conducted with the use of the PRISMA guidelines. Between 1980 to 2020, a comprehensive search was done for any epidemiological data on hepatitis C in Yemen that were conducted in each PubMed, web of Science, Scopus, and Google Scholar. Results: Forty-nine (49) studies were matching the inclusion and exclusion criteria from the four databases. After analyzing these 49 studies we found that in the general group of the population the prevalence was ranged between 0.07%-8.5%. Where the highest prevalence was in pregnant women (up to 8.5%) then the public (up to 6%) then blood donors and children up to 3% and 2.8% respectively. In the intermediate-risk group, hepatitis C virus infection prevalence was ranged between 1.03%-3.5% where all population was from the health department workers. In the high-risk group, the range was between 6.4%-62.7%, and the highest prevalence was recorded in liver disease patients. In the patient's group the range of hepatitis C virus infection prevalence was between 0%-46%, while the sickle cell anemia patients were the lowest prevalence the chronic renal failure patients had the highest prevalence. Conclusion: This systemic review demonstrates a high prevalence of hepatitis C infection in Yemen. There is also a need to expand the scope of research and pay attention to new research groups and other cities to get a more comprehensive idea of the prevalence of the hepatitis C virus in Yemen.