Semiquantitative metabolic parameters showed statistically significant differences among the molecular subtypes of the tumours evaluated. Therefore, there seems to be a relationship between molecular and glycolytic phenotypes.

Adenovirus infection of CD34+ hematopoietic stem/progenitor cells is dependent on the multiplicity of infection (MOI), time of incubation, the volume in which the co-incubation occurs and the presence or absence of growth factors. Studies revealed that a brief co-incubation (1-8 hours), resulted in low levels of transgene expression, suggesting that adenovirus infection of CD34+ cells occurs slowly, and optimal transduction requires a 24 hour exposure to adenovirus. Infection by Ad/beta-gal or Ad/p53 at a MOI of 500:1 provided a high transduction efficiency but inhibited hematopoietic function. However, treatment at a MOI of 50-100 resulted in efficient transduction (10.7-15.7% positive) without detectable toxicity. Secondary proof of adenovirus transgene expression was demonstrated by detection of mRNA for p53 in Ad/p53 infected stem cells. We conclude that a 24 hour exposure to recombinant adenovirus encoding p53 or beta-gal, at a MOI of 50-100 is optimal for in vitro gene transfer to BM cells and has no significant effect on hematopoietic function. Adenovirus-mediated transduction of BM cells can also be modulated by growth factors (IL-3, GM-CSF and G-CSF) with improved gene delivery and maintenance of hematopoietic function. In summary, adenovirus vectors can be used to transiently transduce stem cells, and conditions have been defined to maximize expression and limit inhibitory effects on CD34+ cells. These data support continued investigation of this vector for local cytokine delivery and purging of stem cell products.

Biological factors of bad prognosis were correlated with higher semi-quantitative metabolic values in lymph nodes. Therefore these results appear to reveal biological significance of lymph node (18)F-FDG accumulation.

Summary:be due to the accelerated immune reconstitution (PHA mitogenesis, NK activity, and CD4:CD8 ratio) which is observed following HDT and PSCT as compared to Rapid immune reconstitution is observed following autologous peripheral blood stem cell transplantation ABMT. 7,8 Although there have been limited and conflicting studies on immune reconstitution following PSCT and (PSCT) as compared to autologous bone marrow transplantation (ABMT), although it is depressed compared ABMT,[8][9][10][11] all these studies have reported an impairment in T cell function following transplantation with an inverto that observed in normal individuals. The immune dysfunction occurs despite the restoration of normal sion in the CD4:CD8 cell ratio and a reduced T cell proliferative response. Studies have also suggested that there lymphoid cell numbers and may be associated with the immunologic characteristics of the infused peripheral are differences in immune cell phenotype and function amongst the various apheresis/transplant products. 12,13 blood stem cell (PSC) product. We report herein that the in vitro T cell proliferation and NK activity in PSCThe immune dysfunction that occurs post-transplantation, can reduce the host's ability to control neoplastic products of breast cancer patients are significantly increased following the removal of CD14 ؉ monocytes growth. One possible mechanism of tolerance is the natural suppressor (NS) cell activity which is mediated by cells of (33 ± 2% of the PSC product) by carbonyl iron magnetic cell isolation (CI). In vitro expansion of PSC cells bone marrow (BM) origin and can suppress the proliferative response of lymphocytes in a non-major histocompatcultured for 7-21 days in the presence of interleukin-2 (IL-2) is also significantly increased by depletion of the ibility complex (MHC) restricted manner. 14 Although, the lineage of NS cells is undefined, their activity is detected phagocytic cells. The PHA and IL-2 mitogenic responses, as well as NK activity of the expanded cells, in tissues undergoing intense hematopoietic regeneration, including neonatal spleen, adult BM, and the spleen followwas also significantly increased by the depletion of the phagocytes. In summary, the depletion of phagocytic ing total lymphoid irradiation, cyclophosphamide administration, and tumor growth. 15,16 It is possible that suppressor monocytes from PSC products restores the proliferative and functional properties of T and NK lymphocytes and cells in the BM may migrate to the peripheral blood following growth factor stimulation [17][18][19] and thus be found within may facilitate adoptive cellular therapy, as well as rapid immunologic reconstitution post-PSCT.PSC products. Several studies have also demonstrated that recombinant IL-3 and recombinant granulocyte-monocyte Keywords: monocytes; NK; T cell; suppression; tolerance colony-stimulating factor (rGM-CSF), are efficient at activating bone marrow NS cells, even at very low cytokine concentrations. 17,18 Macrophage-like cells, separable by High-dose chemo...

Here we present the case of a 30-year-old man diagnosed with a dysgerminoma with mediastinal involvement, who developed an isolated chylopericardium during treatment. The purpose of this paper is to review the etiology, diagnosis and new approaches to the treatment of chylopericardium.