Beardless Barb Cyclocheilichthys apogon (Valenciennes, 1842) is a species known from Indonesia, including Borneo, Sumatra, Java, and Madura. This paper provides the first record of C. apogon in the Nyanyi River, one of the major rivers in South Bali, thereby extending the species distribution up to 100 km east from the earlier record. The specimens of C. apogon were characterized as follows: dorsal fin rays 12; anal fin rays 8–9; pectoral fin rays 17–18; lateral line scales 34–35. A description of detailed morfological characters of a specimen are provided.

Oreochromis niloticus (Linnaeus, 1758) is native to Africa, with introductions reported from Bawean Island and Kangean Island, Java Sea (Indonesia). In 2019, four specimens of O. niloticus were captured from an estuary (conservation area) on Masalembo Island. This record is among the first of this species from the middle of the Java Sea. A description of morphological characters of the specimens are provided.

This study aimed to analyze the amount of costs, production volume, revenue, and business profit of CV Tani Subur and analyze the BEP and optimize the use of resources in organic fertilizer production in CV Tani Subur to produce maximum profit. The study was conducted in December 2019 at CV Tani Subur, Sleman. The research method used was quantitative method with cost-volume profit and linear programming data analysis. The results showed cost-volume profit analysis is unsuitable to be used in CV Tani Subur profit planning. The re- event point of CV Tani Subur in 2017 had the highest value and caused losses to CV Tani Subur due to its inability to break even. Furthermore, the analysis of linear programming in CV Tani Subur shows that the company will get optimal results if it only produces cow organic fertilizer as much as 548.3925 tons per year. The advantage achieved if producing organic cow fertilizer which is calculated by calculating the simplex method using POM Quantitative methods for windows version 3 software is IDR. 248,392,500 per year.

Edible bird nest (EBN) is an excellent export commodity from Indonesia and South East Asia countries. To keep the quality assurance of EBN, aflatoxin analysis should be conducted to check its biological hazard. This research was conducted to develop a rapid, sensitive and validated method to detect aflatoxin contamination in EBN. This study showed that in-house AF.2 IAC containing AF.2 monoclonal antibody can be used for EBN sample clean-up. Validation methods were measured using parameters which were recovery (86.5-96.3% for AFB1, 87.8-98.5% for AFB2, 82.8-100.5 for AFG1, 79.8-94.3% for AFG2), intra-day repeatability RSD (⩽ 6.4% for all aflatoxins), linearity (⩾ 0.99997 for all aflatoxins), limit of detection (0.26 ng/g for AFB1, 0.04 ng/g AFB2, 0.26 ng/g for AFG1, 0.03 ng/g for AFG2) and limit of quantification (0.79 ng/g for AFB1, 0.13 ng/g AFB2, 0.81 ng/g for AFG1, 0.09 ng/g for AFG2). These results showed that both recovery test of spiked PBS and recovery test of 3-level aflatoxin concentration spiked IBN were good, thus in-house AF.2 IAC can be used to analyze aflatoxins in EBN. Based on validation parameters such as recovery test, intra-day repeatability, linearity, LOD, and LOQ which had been tested, our method can be used for validated method for aflatoxin analysis in EBN. Further analysis of aflatoxins in edible bird nest will be reported separately.