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<abstract> <p><italic>Bacillus cereus</italic> is reported as a common cause of toxin-induced food poisoning and of contamination in pasteurized human milk donations. As various toxins can be produced by <italic>B. cereus</italic>, the aim of this work was first to investigate the toxigenic potential and profiles of 63 <italic>B. cereus</italic> isolates from Amiens Picardie human milk bank. A comparison to the toxigenic profiles of 27 environmental <italic>B. cereus</italic> isolates harvested in the hospital in which this human milk bank is situated was performed. Toxin gene prevalences were the highest for <italic>nhe (ABC)</italic> and <italic>entFM</italic> followed by <italic>cytK</italic> and <italic>hbl(ACD)</italic>. A 27% prevalence was found for <italic>ces</italic> human milk isolates, which is higher than previous works reporting on pasteurized milk and dairy products. No significant differences could be found between human milk and environmental isolates regarding toxin gene prevalences and/or toxin gene profiles. The second aim was to establish whether a <italic>B. cereus</italic> cross-contamination between human milk and the environment could occur. This was achieved with the help of Fourrier-transform infra-red spectroscopy which enabled the discrimination of 2 main clusters of 11 and 8 isolates, each containing human milk and Amiens Picardie human milk bank environmental isolates. For these two clusters, the time sequence showed that human milk isolates were the first to occur and might have contaminated the milk bank environment as well as other human milk donations. Routinely used on <italic>B. cereus</italic> isolates, Fourrier-transform infra-red spectroscopy could help in rapidly detecting such clusters and in limiting the spread of a <italic>B. cereus</italic> strain that might generate rejection of pasteurized donation by the human milk bank.</p> </abstract>