Staphylococcus aureus is a pathogen associated a different kind of infection. Molecular markers are useful tools to study microbial epidemiology. Twenty two methicillin-resistant S. aureus (MRSA) and 23 methicillin-susceptible S. aureus (MSSA) were studied by mecA gene, SCCmec cassette, Panton Valentine leucocidin (PVL) and spa polymorphism. The clinical data patients were analyzed. MSSA was prevalent in samples different from skin and soft tissue (SST) and in hospitalized patients, whereas MRSA in SST. SCCmec type IV was predominant, followed by type I. Low presence of PVL was found. In MRSA 11 different types of spa were detected, t019 was the most frequent and associated with outpatient, 17 types were found in MSSA and t189 was prevalent. spa t002 was present in MSSA and MRSA. We found 11 types of spa not reported in our country.

e333using PapilloCheck (Greiner Bio-One), which evaluates 24 different HPV genotypes (18 high-risk and 6 low risk).Results: Positivity were 38,37% and 72.1% for cervical cytology and penile lesion biopsies, respectively. Multiple infections account for 41,5% in cervical cytology, and 61.3% in penile lesion biopsies. Of 1311 found viruses in cervical cytilogy, 73,7% were high risk, and of 68 found viruses in penile lesion biopsies 42.6% were high risk.In cervical cytology, positivity (P < 0,0001) and high risk genotypes (P = 0,0087) increased with the degree of cell injury. Moreover, the seven most common genotypes in normal cervical cytology were 16Additionally, hyperkeratosis (P = 0.003), irregular acanthosis (P = 0.003), and papillomatosis (P = 0.037) were associated with HPV in penile lesion biopsies. On the other hand, parakeratosis (P = 0.046), koilocitotic atypia (P < 0.0001), hyperplasia (P = 0.024) and loss of basal polarity (P = 0.024) were more prevalent in multiple infections than in simple.Conclusion: There are differences in positivity, multiple infections, and high risk genotypes prevalence between cervical cytology and penile lesions. Also, proportion of positivity and high risk genotypes increase with degree of cell injury in cervical cytology. Some cellular/hystological changes are significantly associated with HPV and multiple infections in penile lesion biopsies. http://dx.

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Body piercing is a type of self-expression which is based on creating a hole in the skin, subcutaneous tissue or cartilage in order to be able to insert jewellery. The aim was to report severe CA-MRSA infections in two patients, one adult and one child, from different teaching hospitals, in the same period of time and both of them had penetrating implantation as a risk factor. The two isolates were CA-MRSA PVL (+), spa t019, cassette IV and ST30.Referring to the clonal relationship, PFGE showed that both isolates were closely related, indicating that they were probably part of the outbreak. It was not possible to investigate if the piercing setter was the same person in both cases. Mutlu B. Comparison of risk-taking behaviour and frequency of piercing and tattooing among university students. JPak Med Assoc. 2015;65(6):587-592. 3. Purim KSM, Rosario BA, Rosario CS, Guimarães ATB. Piercings in medical students and their effects on the skin. An Bras Dermatol. 2014;89(6):905-910. Méndez EA, et al. J Bacteriol Parasitol, Vol.10 Iss.2 No:356 18. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995;33(9):2233-2239. Méndez EA, et al.

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