6-thioguanine (6-TG) is an antineoplastic, nucleobase guanine, purine analog drug belongs to thiopurine drug-family of antimetabolites. In the present study, we report an experimental approach towards interaction mechanism of 6-TG with human serum albumin (HSA) and examine the chemical stability of HSA in the presence of denaturants such as guanidine hydrochloride (GdnHCl) and urea. Interaction of 6-TG with HSA has been studied by various spectroscopic and spectropolarimeteric methods to investigate what short of binding occurs at physiological conditions. 6-TG binds in the hydrophobic cavity of subdomain IIA of HSA by static quenching mechanism which induces conformation alteration in the protein structure. That helpful for further study of denaturation process where change in secondary structures causes unfolding of protein that also responsible for severance of domain III from rest of the protein part. We have also performed molecular simulation and molecular docking study in the presence of denaturating agents to determine the binding property of 6-TG and the effect of denaturating agents on the structural activity of HSA. We had found that GdnHCl is more effective denaturating agent when compared to urea. Hence, this study provides straight evidence of the binding mechanism of 6-TG with HSA and the formation of intermediate or unfolding transition that causes unfolding of HSA.

Locusts are the most dangerous agricultural pests. They are belonging to family Acrididae. Gregarious locusts travel in swarms from one location to another in adult form. The desert locust, Schistocerca gregaria (Forskal), is one of the grasshopper species that cause crop damage and can fly up to 150 km in the direction of the wind. The present state of knowledge on its biological regulation employing microbes and plant extracts is discussed. Metarhizium flavoviride was among the first fungi to be identified in the laboratory and field as a bio-control agent against desert locust. Following extensive investigation, with integrated pest management stratedies using these bio-controls would be a viable option for controlling desert locust infestations. Against the desert locust, IPM (Integrated Pest Management) approaches that emphasize the successful combination of chemical and biological insecticides with prediction and monitoring technology have been encouraged. Recent experimental investigations and researches are mainly focusing on identifying viable answers through financial collaboration between governmental and non-governmental organizations. The authors highlighted the loss in the agricultural sector due to desert locust infestation, as well as its sophisticated control and management solutions, after evaluating publications from numerous journals, magazines, and symposia.

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