The aim of this study was to evaluate the oxidative status in crossbred cows with subclinical mastitis and concurrent metabolic and infectious diseases. The crossbred cows were groped asGp-C (n=6) as controls,Gp-M (n=6) with clinical mastitis ,Gp-TS consists cows affected withstenosis of teat,Gp-RA with ruminal acidosis,Gp-ND with diarrhea along with dehydration and loss of appetite, Gp-RTIwith coughing, fever, decreased appetite, varying degrees of dyspnea, GpRB with a history of failure to conceive after at least two successive inseminations. oxidative stress parameters likecatalase, SOD, GPx, GSH and malondialdehyde were estimated spectrophotometrically. The antioxidant enzymes like catalase, super oxide dismutase (SOD), Glutathione Peroxidase (GPx) levels were significantly declined and the non enzymatic parameters reduced glutathione (GSH) and malondialdehyde (MDA) are significantly elevated in affected animals than in healthy animals. The results of the oxidative stress parameters indicate imbalance of antioxidant profile and oxidative stress in the animals with various disorders and previous illness along with subclinical mastitis.