The oil-soft wax fraction of the microcrystalline wax, extracted from the Bombay High crude tank bottom sludge (Indian origin), has been characterized using gas liquid chromatography (GLC), 1H and 13C nuclear magnetic resonance (NMR), and gas liquid chromatography−mass spectroscopy (GLC−MS). A series of saturated acyclic isoprenoid hydrocarbons have been identified in this wax. These structures have been confirmed by recording GLC-MS spectra of the oil-soft wax fraction at reduced ionization potentials of 30 and 20 eV and also recording the spectra of two known available standards, namely, pristane and phytane.