The budding yeast cortical proteins Boi1 and Boi2 were previously implicated in polarized growth and in inhibition of cytokinesis as part of the NoCut abscission checkpoint. This report establishes an essential role of Boi1 and Boi2 in vesicle exocytosis during bud growth, whereas Boi2 (but not Boi1) is required for abscission inhibition.

Boi1 and Boi2 (Boi1/2) are budding yeast plasma membrane proteins that function in polarized growth, and in cytokinesis inhibition in response to chromosome bridges via the NoCut abscission checkpoint. How Boi1/2 act in these two distinct processes is not understood. We demonstrate that Boi1/2 are required for a late step in the fusion of secretory vesicles with the plasma membrane of the growing bud. Cells lacking Boi1/2 accumulate secretory vesicles and are defective in bud growth. In contrast, Boi2 is specifically required for abscission inhibition in cells with chromatin bridges.