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The biosensor consists of an optical sensing system and an optoelectronic data acquisition system. The sensor’s optical system consists of a biochemically functionalized polymer optical fiber (POF-Plastic Optical Fiber) based on Field Evanescent technology. The Evanescent Field technique has been widely adopted in sensing and in this project, it was obtained by bending the fiber in a “U” shape, aiming to increase the sensitivity of the biosensor, through the contact of the curved sensor part with the sample biological. A data acquisition system was developed through an optoelectronic project aiming to increase the sensitivity when compared to a commercial equipment acquisition system. This work presents a biosensor for the detection of Escherichia coli based on an evanescent field with a polymer optical fiber linked to the analog signal acquisition system through an optoelectronic system developed. The interaction investigation of antibodies and antigens in Escherichia coli for computational methods was carried out in order to obtain information about the action of the antibody and in future steps applied in the validation of the diagnostic method.

Aims: This research aims to report the genome sequence of the Bacillus sp. strain AN2, isolated from agricultural soil from Rio de Janeiro, Brazil. Place and Duration of Study: Laboratory of Environmental Biotechnology, Western Rio Janeiro State University in Brazil, between January 2019 and December 2019. Methodology: High-quality genomic DNA was extracted using a GenElute Bacterial Genomic DNA kit. The Nextera XT DNA Library Prep Kit was used for genomic library construction. Paired-end sequence reads were generated by an Illumina MiSeq instrument with the 600 cycles MiSeq Reagent Kit v3. Sequence data were assembled with A5-MiSeq pipeline software and the contigs were annotated by Rapid Annotation using Subsystems Technology (RAST). Results: The obtained genome sequence of Bacillus AN2 included 21 contigs with a calculated size of 3,681,081 bp in length. The G + C content for the draft genome is 41.4%. A total of 3824 coding sequences (CDS) were predicted and encoded at least 88 tRNAs.