Essential oil of Piper betle leaves in Quang Nam-Da Nang do obtain via the steam distillation method with oil collection efficiency reaching 0.83%. The chemical composition of the essential oil from Piper betle leaves in Quang Nam-Da Nang is determined via the GC-MS method that includes 21 components (99.68%), among which the main components are eugenol acetate (32.33%), eugenol (24.56%), 4-allyl-1,2-diacetoxybenzene (19.8%), germacrene D (5.98%), caryophyllene (3.32%), and γ-muurolene (2.45%). The essential oil of Piper betle leaves in Quang Nam-Da Nang also showed moderate anti-acetylcholinesterase activity with IC50 (µg/mL) = 65.94±5.96 and moderate nitric oxide suppressing activity with IC50 (µg/mL) = 46.69±3.57.

Cardiovascular diseases are one of the leading cause of mortality and morbidity worldwide. Although many efforts have been made in the drug discovery and development process through decades, the number of approved drugs has been declining. In the recent years, soluble epoxide hydrolase (sEH) has been considered as promising target for drug development since inhibiting sEH function would prevent the formation of arterial thrombosis. Paederia foetida L. is a folk medicine distributed commonly in Vietnam which is well known for its uses in the treatment of various diseases. This study conducted in silico assessment of 21 isolated compounds from leaves of Paederia foetida L. against sEH enzyme for potential inhibition activity. Obtained results demonstrated that compound 4 and 9 could be potent, safe and novel inhibitors based on docking conformation and druglikeness properties.

Piper longum fruits oil in Binh Dinh, Vietnam was obtained via steam distillation with oil collection efficiency reaching 1.01%. The chemical compositions of the essential oil from Piper longum fruits in Binh Dinh, Vietnam is determined via the GC-MS method that includes 35 components (99.68%), among which the main components are caryophyllene (10.78%), 3-heptadecene (9.95%), zingiberene (9.54%), germacrene D (8.96%), pentadecane (8.76%), heptadecane (8.73%), β-bisabolene (5.98%), humulene (5.80%), (E)-5-tetradecene (2.73%), α-bisabolene (2.47%), tridecane (2.35%). The essential oil of Piper longum in Binh Dinh, Vietnam also showed weak anti-acetylcholinesterase activity with IC50 (µg/mL) = 164.55±13.79 compared to galantamine as a positive control and potent nitric oxide suppressing activity with IC50 (µg/mL) = 13.02±0.29 compared to dexamethasone as a positive control.