A cross-sectional study was conducted among 171 doctors and nurses in a National Dermatology hospital using the Karasek's Job Content Questionnaire which has been validated in Vietnamese (JCQ-V), to assess the prevalence of occupational stress and to explore the association with some associated factors among them. The result showed that doctors and nurses with occupational stress accounted for 6.4%. This proportion was higher among nurse compared to doctor (8.0% vs. 2.2%); among those with diploma literacy compared to bachelor and above (10.6% and 2.3%). This rate was also higher in health workers under 30 years old (12.9%), health workers under 5 years at work (12.1%), working night shift from 3-4 nights (33.3%), temporary employment (12.8%), heavy workload occasionally (12.5%), and working hard occasionally (17.2%) compared to those in the comparison groups with p value <0,05. This prevalence concentrated in some departments such as surgery (11.9%), internal medicine (6.7%), dermatology, and others (1.5%). The study has not found the significant association between the prevalence of occupational stress and heavy workload and skill level. Therefore, it is essential for hospital should conduct screening all doctors, nurses, and medical staffs to identify subjects having occupational stress and give appropriate intervention.

• A simple and effective evaporation combined extrusion method to prepare curcuminoidsloaded nano phytosome is successfully applied. • The physically stable phytosomal nanoparticles of curcuminoids were formed at a spherical shape with good size (~ 180 nm), a narrow size distribution (PDI < 0.2), high complexation rate (~87%, 95%, and 90% for bisdemethoxycurcumin, demethoxycurcumin, and curcumin, respectively) and high loading capacity of curcuminoids. • The interaction between curcuminoids and phospholipid by a hydrogen bond was confirmed by differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), fourier transform infrared (FT-IR), and 1 H nuclear magnetic resonance (1 H-NMR). • Curcuminoids-loaded nano phytosomes showed significantly higher cellular uptake and in vitro cytotoxicity profile on breast cancer cell line compared to raw curcuminoids indicating the potential of phytosomal nanoparticles as a promising delivery system for natural herbal phytoconstituents for anticancer applications.

No abstract

• A rapid and selective ultra performance liquid chromatography (UPLC) with detector MS/ MS to quantify phyllanthin in rat plasma in 1.5 minutes is described. • Phyllanthin is extracted from a small volume of rat plasma (100 µl) by means of liquid-liquid extraction method. • The validated method was successfully applied to pharmacokinetic studies of oral administration of different phyllanthin formulations to rats. • Phyllanthin from complex with phospholipid showed significantly higher AUC, C max and a longer half-life than that from raw Phyllanthus amarus extract indicating higher bioavailability of the phyllanthin-phospholipid complex.