Osteomas are benign tumors which are composed of mature compact or cancellous bone. They can be either peripheral, central or extraskeletal. The peripheral osteoma arises from surface of the bone (periosteal) whereas the central osteoma arises from the bone medullary (endosteal) and the extra-skeletal soft tissue osteoma usually develops within the muscle. Osteomas are most commonly found in the skull and facial bones. Multiple osteomas may be associated with Gardner’s Syndrome. These lesions are usually painless and recurrence is uncommon after local excision. In this case report clinical, radiographic findings and treatment of a 24-year-old male patient with peripheral osteoma in the anterior mandible are presented.

Objective: KRAS mutation attenuates treatment effect of cetuximab. The purpose of this study was to develop a rapid and specific method to detect KRAS mutation at codon 12 using novel gene amplification methods.Study Design: Previously, loop-mediated isothermal amplification (LAMP) assay was developed to amplify copies of particular DNA sequence. LAMP assay is rapid and specific method rather than polymerase chain reaction process. We modified LAMP assay by using peptide nucleic acid (PNA) to suppress an amplification of wild-type allele.Results: We successfully developed modified LAMP assay in several cultured cell lines. By using this assay, we can amplify DNA fragment of mutated KRAS gene within 20 minutes. Primers bracketing the nucleotides of KRAS codon 12 were designed so that all possible alleles would be amplified by this assay.Conclusions: The modified LAMP assay is a rapid, and highly sensitive detection assay for cancer mutations.

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ÖZETPeriferal dev hücreli granülom (PDHG) oral bölgenin sık izlenen dev hücreli bir lezyonudur. PDHG'ler gerçek bir neoplazmı temsil etmezler. Etyolojileri çok açık olmayan bu lezyonların, travma veya lokal irritasyonlara bağlı gelişen reaktif bir lezyon olduğu düşünülmektedir. PDHG'lere kadınlarda erkeklere oranla daha sık, mandibulada ise maksilladan daha fazla rastlanılır. Gingiva veya dişsiz alveolar kret üzerinde kırmızı, kırmızı-mavi nodüler kitle şeklinde görülürler. Bu olgu raporunda, 48 yaşında erkek hastada görülen, maksilla sol santral kesicisağ kanin kesici dişler ve mandibula sağ santral kesici-sol kanin kesici dişler bölgesinde lokalize, yüzde asimetriye neden olan büyük boyutlu PDHG'ler sunulmuştur. Multipl PDHG vakasının radyolojik, klinik, histopatolojik bulguları ile birlikte teşhis, tedavi ve 6 aylık takibi rapor edilmiştir.Anahtar Kelimeler: Periferal dev hücreli granülom, mandibula, maksilla, multipl lezyon SUMMARYPeripheral giant cell granuloma (PGCG) is the most common oral giant cell lesion. PGCG presumably does not represent a true neoplasm. PGCG is believed to be stimulated by local irritation or trauma besides the causing of PGCG isn't known exactly. PGCG affects women more frequent than men and mandible more often than maksilla. It is usually seen as red or redhiss-blue, noduler mass on the gingiva or edentulous ridge. In the case report; multiple large PGCGs of 48 years old male patient causing facial asymmetry, localizated on the gingiva including the alveolar ridge of his maxillary left central incisor-right canin region and mandibular right central incisor-left canin region is presented. This case report contains radiological, clinical, histopathological findings, as well as diagnosis, treatment, follow-up of 6 months after totally excision of multiple PGCSs.