Research on the antibacterial assay of the α-pinene and turpentine oil in hand sanitizer gel formulation has been carried out. Antibacterial activity assay using the disc diffusion method showed that turpentine oil has the highest antibacterial activity with a inhibition zone of 13.8 mm against Staphylococcus aureus and 8.83 mm against Escherichia coli, whereas α-pinene has the highest antibacterial activity with a 2.2 mm inhibition zone against S. aureus and 2.34 mm against E. coli.