The regulatory and catalytic subunits of cAMP-dependent protein kinase (PKA) were coexpressed within the same bacterial cell using a polycistronic bacterial T7 expression vector encoding Chinese hamster cDNAs for the type I regulatory (RI) (13,14) and the BL21(DE3)/Acya strain was prepared by P1 transduction as described (6). The pVEX-11 vector was a generous gift of V. Chaudhary (National Institutes of Health, Bethesda, MD) (15). BL21(DE3) cells were grown in LB broth, BL21(DE3)/pLysS and BL21(DE3)/pLysE were grown in LB broth with chloramphenicol (15 ,ug/ml), and all strains transformed with the pVEX constructs were grown in LB broth supplemented with ampicillin (50 ,ug/ml) (16).Preparation of the Polycistronic Expression Vector. A 1.1-kb Xba 1-HindIII fragment excised from pT7Ca (a gift from R. Maurer, University of Iowa) (7) was blunt-ended by incubation with the large fragment of DNA polymerase I (Klenow fragment) (17). This Ca fragment, containing a ribosome binding site (Shine-Dalgarno sequence) followed by the full-length Chinese hamster Ca cDNA, was blunt-end cloned (17) into the blunt-ended HindIII site of pVEXRI (6). An Asp718 site was generated in the vector by replacing the HindIII-Nde I fragment with the oligodeoxynucleotide seAbbreviations: PKA, cAMP-dependent protein kinase; IPTG, isopropyl -D-thiogalactopyranoside.

Leiomyosarcomas are rare malignant tumors of the kidney. They may arise from the renal capsule, renal vein, renal pelvic musculature or renal parenchyma. Renal pelvis is an uncommon site of occurrence, with around 10 cases reported in the literature so far. Here we present a 60-year-old male who presented with increased urinary frequency, lower limb weakness, anorexia and weight loss. Imaging showed a right renal mass. A renal cell carcinoma was suspected clinically. A right nephrectomy was performed, which showed a large circumscribed mass in the hilar region. Histology revealed a tumor mass arising from the renal pelvis. The tumor was composed of spindle cells arranged in fascicles. Immunohistochemistry showed tumor cells to be positive for smooth muscle actin (SMA) and desmin (Des) and negative for cytokeratin (CK), HMB 45, CD117 (C-kit), and CD34. That confirmed the diagnosis of leiomyosarcoma.

Complete Androgen insensitivity syndrome is a disorder of hormone resistance characterized by a female phenotype with an XY karyotype and testes producing age-appropriate normal or higher concentrations of androgens. We present a case of 26 year old, unmarried phenotypically female, with left inguinal swelling and amenorrhea. MRI finding revealed bilateral inguinal masses, uterus cervix was not visualized and hypoplatic vagina was noted. Karyotyping revealed her genotype as 46 XY. Hormonal investigation showed testosterone, estradiol and LH were increased and FSH was within normal limits. Patient underwent laparoscopic bilateral gonadectomy with left open hernia repair. Histopathology examination revealed hamartomatous nodule, sertoli cell adenoma, leydig cell hyperplasia, which are more pronounced as age advances as result of absent activity of androgen. Fallopian tube, underdeveloped vas deference, Wolffian/Müllerian cysts lined by cuboidal epithelium was also noted which may be reminiscent of Wolffian/ Müllerian structure. Immunostaining for PLAP and CD 117 were negative. The clinical, MRI, laboratory and histopathology findings confirmed diagnosis of complete androgen insensitivity syndrome.