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Diabetes Mellitus (DM) can result in chronic non-healing wounds ultimately leading to lower limb amputations. Studies have shown haired punch grafts can accelerate wound closure when compared with non-haired grafts, however the contribution of different populations of fibroblasts from haired skin to wound healing under elevated glucose levels and the effects of high glucose concentrations (as seen in DM) on these cells are unknown. We aimed to determine the effect of basal (1g/L) and high glucose (4.5g/L) concentrations on three different fibroblast subpopulations cultured from haired skin (papillary dermis (DF), hair follicle dermal sheath (DS) and hair follicle dermal papilla (DP)). Cell counts showed proliferation of all cell subtypes decreased in high glucose compared to basal levels in the presence of 10% serum. Ki-67 positive cells were also reduced in high glucose treated cells compared to basal. The

TRP ion channels are non-specific cationic channels with 6 transmembrane domains, most of them are permeable to Ca 2þ ions. Primarily, the multimodal thermosensitive members of the TRP family, TRPV1-4, TRPA1, and TRPM8, are proven to play role in skin sensory functions. In addition to sensory neurons in the skin, some of our previous work has also described the presence of sensory TRP channels on a number of non-neural cells and has been implicated in the physiological and pathological biological processes of keratinocytes and skin appendages (eg sebaceous gland, hair follicles). In this project, we investigate the role of keratinocytes and their heatsensitive TRP channels in the development of itch and related pruriceptive signaling. Primary normal human epidermal keratinocytes (NHEK) isolated from surgical specimens and We studied the expression (RNASeq, Q-PCR, ELISA) and function (intracellular Ca 2þ measurements) of TRP channels and other molecules involved in pruritic signaling in normal human epidermal keratinocytes (NHEKs). Expression of several sensory TRP channels was detected on NHEKs, among which TRPV3 and TRPV4 wee expressed at highest level. Their functionality was confirmed by Ca 2þ measurements. In addition to TRP channels, keratinocytes also expressed a number of receptors and mediators that may play a role in the development of pruritus or pruritus-related dermatological disorders (eg, proteinase-activated receptor 2, TLR3, histamine H1 receptor, etc.). Subsequently, we investigated the role of TRP channels in the signaling pathways related to these potentially pruritogenic molecules in NHEKs. According to our results, activation of TLR3 upregulated TRPV3 and enhanced subsequent Ca 2þ signals, which may contribute to other TLR3 evoked responses. Based on our results, it appears that keratinocytes may play a role in the transduction of certain itch-inducing signals.

Jelen tanulmány arra a kérdésre keresi a választ, hogy az orvosok milyen kiégési értékekkel rendelkeznek, és ezek milyen összefüggésben állnak az esetükben mért munkahelyi elégedettséggel, szervezeti elkötelezettséggel és motivációval. Vajon az az orvos, aki elégedett a munkájával, elkötelezett a szervezet iránt és megfelelő motivációs szinttel rendelkezik, valóban alacsonyabb kiégési értékeket fog mutatni? Milyen lehet az összefüggés e három tényező között? Különböző szakterületen dolgozó orvosok különböző értékeket fognak mutatni? Kutatásunkban négy csoportot vizsgáltunk: belgyógyászok, háziorvosok/házi gyermekorvosok, traumatológusok és pszichiáterek csoportját. Az eltérő típusú munka és betegszám elég okot adhat a különbségekre. Vizsgáltuk az életkor, a nem és az ügyelet vállalásának befolyását a kiégés szindróma kialakulására.