This study assessed knowledge, attitudes and perceptions of various stakeholders in Grenada regarding genetically modified organisms and the release of genetically modified mosquitoes. The study adopted a cross sectional design with a study population that comprised of members from seven stakeholder groups of civil society in Grenada. The sample population was obtained through a snowballing strategy and data collection for the study consisted of a semi-structured interview. Data was managed through the collection and reviewing of data from transcribed interview notes, as well as observations and interpretations made during the field collection. Interview recordings were analyzed to identify emerging themes. These themes were ranked according to the frequency with which they appeared, and main concepts identified by linking related themes. Most groups supported the use of genetically modified mosquitoes against Zika, but there were several varying concerns, including environmental worries and the impact of these organisms on humans. Many questioned the characteristics of Zika itself, and some believed Zika is a man-made virus created in a lab. Others doubted the link of Zika virus to microcephaly and other birth defects, and some were unsure if Zika virus is transmitted by mosquitoes. There is a wide range of differing knowledge, attitudes, and perceptions towards genetically modifying technology in general and towards mosquitoes in response to Zika.