Spore suspensions of Penicillium spinuloswn have been prepared containing over 99.0 per cent single spores in an even distribution. About 80 per cent of the spores were able both to produce germ tubes and to form colonies in a roll‐tube. A peptone powder containing an even distribution of spores has been prepared. The viable count of the powder did not show a significant fall during 5 months storage. The suspension has been successfully used to study the sporicidal and fungistatic activities of chlorocresol solutions.